The process of proteomic analysis begins with cell lysis, which is then followed by purification. For efficient protein solubilization, lysis buffers typically contain detergents and other reagents. Here, we present a curated selection of commonly used lysis buffers, kits, and related biochemicals.
Given the wide variety of sample sources and proteins, providing a universal solution is not feasible. One approach is to try ready-to-use buffers that have been proven effective across most organisms. Alternatively, specialized kits exist for specific proteins or organisms, such as the Minuteâ„¢ kit optimized for bacteria.
For customized lysis solutions, we offer a comprehensive range of protease inhibitors, detergents, and other high-purity biochemicals tailored for biological applications.
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### RIPA Lysis and Extraction Buffer
#### For Optimal Lysis of Adherent & Suspension Cells - Compatible with Purification
This complete lysis buffer is designed to release cytoplasmic, membrane, and nuclear proteins from both adherent and suspension-cultured mammalian cells. The RIPA lysis buffer is highly versatile and works well with numerous applications, including reporter assays, protein assays (BC Assay), immunoassays, and various protein purification techniques. It excels particularly for nuclear, cytoplasmic, and mitochondrial proteins, while cytoskeletal and extracellular region proteins tend to be more soluble in urea.
Note that the RIPA Lysis Buffer does not contain protease inhibitors but is fully compatible with a variety of individual protease inhibitors and cocktails.
**Protein Digestion**
**Mass Spectrometry-based Protein Identification and Analysis (HPLC-MS)**
**RIPA Lysis and Extraction Buffer HG4361 100mL**
Contents: 25mM Tris, 150mM Sodium Chloride, 1% NP-40, 1% Sodium Deoxycholate, 0.1% SDS, pH 7.6
Search RIPA buffers online.
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### Minuteâ„¢ Bacterial Total Protein Extraction Kit
#### Rapidly Extract Total Proteins from Cultured Bacterial Cells for Applications Such as SDS-PAGE, Immunoblotting, ELISA, etc.
The Minuteâ„¢ Bacterial Total Protein Extraction Kit comprises optimized denaturing cell lysis buffers and protein extraction filter cartridges with 2.0 mL collection tubes. Designed to swiftly extract denatured proteins from bacteria, this kit includes enough materials to extract total proteins from a 100 mL *E. coli* culture.
Comparison of Protein Profiles Extracted by Minute Kit and RIPA Buffer
Lanes 1, 2, and 3 represent proteins extracted by the Minute kit, RIPA buffer, and the insoluble fraction of RIPA extraction, respectively.
**Minuteâ„¢ Bacterial Total Protein Extraction Kit SB-004 50 Preps**
Contents: 25 mL bacterial lysis buffer A + 2.5 mL buffer B + 50 protein extraction filter cartridges (500 µL) + 50 collection tubes with caps
Search Minuteâ„¢ extraction kits online.
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### Protease Inhibitors
Protease inhibitors are molecules that bind either reversibly or irreversibly to proteases, thereby inhibiting their activity. Most inhibitors are specific to one or two types of proteases.
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#### Serine Proteases
| **Name** | **Description** | **Catalog #** | **Quantity** |
|----------------|---------------------------------------------------|---------------|--------------|
| AEBSF HCl | >98% | 401070 | 100 mg |
| | | 401071 | 1 g |
| Aprotinin | Powder, bovine lung 6300 KIU/mg, 3.5 pH.Eur.U/mg | BXH470 | 100 mg |
| | Solution, 200,000 KIU/mL – Pharma Grade | 24431A | 50 mL |
| PMSF | >99.9% | 147376 | 5 g |
| | | 24431A | 50 mL |
| Benzamidine | Hydrochloride, anhydrous, >99% | 003055 | 100 g |
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#### Metalloproteases
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| EDTA | Disodium Salt >99.5%, Molecular Biology Grade | 036290 | 1 kg |
| EGTA | >99 % | 10075A | 25 g |
| Phenanthroline | Anhydrous, >99 % | E4414E | 25 g |
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#### Cysteine Proteases
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| E-64 | >99% | 789581 | 5 mg |
| | | 789582 | 25 mg |
| Iodoacetamide | >99 % | 020483 | 25 g |
| Leupeptin | Hemisulfate >97%, Synthetic | 201736 | 25 mg |
| NEM | N-Ethylmaleimide >98 % | 04474B | 25 g |
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#### Aspartate Proteases
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| Pepstatin | >90 % | 827752 | 25 mg |
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#### Protease Inhibitor Cocktails
| **Name** | **Description** | **Catalog #** | **Quantity** |
|--------------------------------|-------------------------------------------------------|---------------|--------------|
| Protease Inhibitor Cocktail I | General Use (AEBSF HCl 500 µM, Aprotinin 150 nM, etc.) | WT0900 | 1 flask |
| Protease Inhibitor Cocktail II | for Bacteria (AEBSF HCl 20 mM, Bestatin 1.7 mM, etc.) | WT8260 | 1 flask |
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### Detergents
Detergents are used to separate membrane proteins from membranes and to open intracellular compartments such as lysosomes. Some detergents stabilize proteins in hydrophilic environments.
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#### Non-Denaturing
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| CHAPS | >98 % | 33351A | 10 g |
| Triton X-100 | 10% solution, Carbonyl and Peroxide Free (>99%) | BT84L0 | 50 mL |
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#### Denaturing
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| SDS | 20% solution, 0.2 micron filtered, MB Grade | 896826 | 500 mL |
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### Others
| **Name** | **Description** | **Catalog #** | **Quantity** |
|------------|--------------------------------------------------|---------------|--------------|
| Guanidine HCl | >99.5 %, Chaotropic Agent | 018380 | 500 g |
| D-Glucose | Anhydrous, >99.9% | 40250B | 500 g |
| Glycerol | >99.8%, Sterile | 047624 | 500 mL |
| EDTA | Disodium Salt, >99.5% | 036290 | 1 kg |
| DTT | >99 % | 054721 | 5 g |
| Urea | >99.5%, Ammonia-, Cyanate-Free | 031904 | 1 kg |
| Tris | 1M pH 7.5, Sterile Solution | N13713 | 1 L |
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### Know More:
- Search protease inhibitors and detergents online.
- Contact us at +33 4 70 03 73 06 for personalized support.
This compilation aims to provide researchers with essential tools for optimizing their proteomic workflows.
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