Indoor cultivation of Hericium erinaceus methods are bottle planting and open type, culture materials are sawdust, cottonseed skin, etc.: The suitable temperature for the growth of Hericium erinaceus mycelium is 22～28°C; the suitable temperature for fruit body growth is 18 ~ 22 °C, the temperature is too low, the fruit body grows slowly. Mycelium has a humidity requirement of 60%, and the fruit body has a humidity requirement of 85 to 95%.
First, the cultivation of Hericium erinaceus should be carried out first. The strains are separated by spore separation and tissue separation. Spore separation is the collection of spores that are released after mature Hericium erinaceus under sterile conditions, as a raw material for the cultivation of hyphae. Tissue separation is to take a fresh and young fruiting body, put it into the inoculation box, cut the mushroom body under aseptic conditions, take a piece of tissue that is full of middle and full of flesh, about the size of rice, and put the slant medium in the test tube. on. The culture medium is made of peeled potato and western vegetables, and the mixture is made of glucose. The ratio is 200:20:20, 1000 g of water is added, and the pH is adjusted to 5.5 to 6, and then put into glass test tubes respectively. Disinfected and autoclaved to form inclined surfaces. After inoculation, the test tube was incubated in an incubator at 25°C for 3 days, and the inoculation block began to germinate. When the silkworm grows over the surface of the culture medium for 20 to 25 days, the well-cultured, non-hybrid-free culture can be selected and the bacteria can be selected. If the silk is over the slant of the test tube culture medium, cultivating species can be started.
Second, the cultivation of cultivated species Cultivated species culture materials can be used sawdust (without aromatic oil or insecticide) or cotton skin, etc., the proportion of ingredients can be selected:
(1) sawdust 78%, bran 20%, gypsum l%, sugar 1%;
(2) 98% cotton husk, 1% gypsum, 1% sugar;
(3) 80% of fresh lees, 10% of bran, 9% of yttrium, 1% of gypsum powder (inorganic salt solution, formula: ammonium sulfate 3%, potassium dihydrogen phosphate 1%, magnesium sulfate 0.1%, Adjust to pH 4 with lactic acid.
(4). Add 60 to 65% water to the medium and mix well. When hand-kneading, there is no water drop between the fingers. It is then bottled to the shoulder of the bottle and sterilized at a pressure of 14.7 newtons for 1 hour. After sterilization, use a sterile operation to pick a piece of slant bacterium and put it into the medium bottle so that the temperature reaches 20-25°C, the relative humidity of the air reaches 60-65%, and the tampon is stuffed. About 30 to 40 days, the mycelium grows over the culture material and forms a pellicle. When the pellicle primordia near the tampon is removed, remove the tampon.
Third, the development of fruiting bodies Open cultivation of Hericium erinaceus system will be broad-leaved sawdust under high pressure sterilization, or boil in the pot for 20 minutes, remove and drain the water, cool, directly after mixing about 40% of the bacteria in the bottle After mixing, put it in a plastic bag or wrap it with a film. The bag mouth or wrap mouth is filled with tampon and ventilated, and cultured at a low temperature below 10° C. After being grown into a bacterial mass, the film is peeled off, and then the culture is continued at natural temperature, and the monkey can be grown several months later. Head mushroom. This method is similar to bottle production, but it can save bacteria and bottles and can be produced on a larger scale. The bottle-grown Hericium erinaceus is kept at 18-20°C after the tampon is opened. If the temperature is too high, doors and windows are closed at noon. Ventilation is performed when the temperature is low in the morning and in the evening. If the temperature is low, it can be ventilated at noon. The indoor humidity is kept at 85 to 95%. When it is too low, water can be poured on the floor and it can be kept moist. At the same time, it can be sprayed in the space for 3 to 4 times per day. The fruiting body can be formed after half a month. Compared with sawdust and cotton hulls as culture materials, cotton rind has the characteristics of rapid bacterial growth, less germs, and early formation of fruiting bodies. It forms fruit bodies about half a month earlier than sawdust.
Fourth, the harvesting fruiting bodies began to shrink and age, stop growing and development, and the surface turned from white to brown, which can be harvested and air-dried. If eaten, it can be harvested before spores are released, eliminating the bitterness of spores. The bottle-grown Hericium erinaceus can harvest three bottles per bottle under the same management conditions as above, and the remaining mycelia can be used for pharmaceutical purposes.
First, the cultivation of Hericium erinaceus should be carried out first. The strains are separated by spore separation and tissue separation. Spore separation is the collection of spores that are released after mature Hericium erinaceus under sterile conditions, as a raw material for the cultivation of hyphae. Tissue separation is to take a fresh and young fruiting body, put it into the inoculation box, cut the mushroom body under aseptic conditions, take a piece of tissue that is full of middle and full of flesh, about the size of rice, and put the slant medium in the test tube. on. The culture medium is made of peeled potato and western vegetables, and the mixture is made of glucose. The ratio is 200:20:20, 1000 g of water is added, and the pH is adjusted to 5.5 to 6, and then put into glass test tubes respectively. Disinfected and autoclaved to form inclined surfaces. After inoculation, the test tube was incubated in an incubator at 25°C for 3 days, and the inoculation block began to germinate. When the silkworm grows over the surface of the culture medium for 20 to 25 days, the well-cultured, non-hybrid-free culture can be selected and the bacteria can be selected. If the silk is over the slant of the test tube culture medium, cultivating species can be started.
Second, the cultivation of cultivated species Cultivated species culture materials can be used sawdust (without aromatic oil or insecticide) or cotton skin, etc., the proportion of ingredients can be selected:
(1) sawdust 78%, bran 20%, gypsum l%, sugar 1%;
(2) 98% cotton husk, 1% gypsum, 1% sugar;
(3) 80% of fresh lees, 10% of bran, 9% of yttrium, 1% of gypsum powder (inorganic salt solution, formula: ammonium sulfate 3%, potassium dihydrogen phosphate 1%, magnesium sulfate 0.1%, Adjust to pH 4 with lactic acid.
(4). Add 60 to 65% water to the medium and mix well. When hand-kneading, there is no water drop between the fingers. It is then bottled to the shoulder of the bottle and sterilized at a pressure of 14.7 newtons for 1 hour. After sterilization, use a sterile operation to pick a piece of slant bacterium and put it into the medium bottle so that the temperature reaches 20-25°C, the relative humidity of the air reaches 60-65%, and the tampon is stuffed. About 30 to 40 days, the mycelium grows over the culture material and forms a pellicle. When the pellicle primordia near the tampon is removed, remove the tampon.
Third, the development of fruiting bodies Open cultivation of Hericium erinaceus system will be broad-leaved sawdust under high pressure sterilization, or boil in the pot for 20 minutes, remove and drain the water, cool, directly after mixing about 40% of the bacteria in the bottle After mixing, put it in a plastic bag or wrap it with a film. The bag mouth or wrap mouth is filled with tampon and ventilated, and cultured at a low temperature below 10° C. After being grown into a bacterial mass, the film is peeled off, and then the culture is continued at natural temperature, and the monkey can be grown several months later. Head mushroom. This method is similar to bottle production, but it can save bacteria and bottles and can be produced on a larger scale. The bottle-grown Hericium erinaceus is kept at 18-20°C after the tampon is opened. If the temperature is too high, doors and windows are closed at noon. Ventilation is performed when the temperature is low in the morning and in the evening. If the temperature is low, it can be ventilated at noon. The indoor humidity is kept at 85 to 95%. When it is too low, water can be poured on the floor and it can be kept moist. At the same time, it can be sprayed in the space for 3 to 4 times per day. The fruiting body can be formed after half a month. Compared with sawdust and cotton hulls as culture materials, cotton rind has the characteristics of rapid bacterial growth, less germs, and early formation of fruiting bodies. It forms fruit bodies about half a month earlier than sawdust.
Fourth, the harvesting fruiting bodies began to shrink and age, stop growing and development, and the surface turned from white to brown, which can be harvested and air-dried. If eaten, it can be harvested before spores are released, eliminating the bitterness of spores. The bottle-grown Hericium erinaceus can harvest three bottles per bottle under the same management conditions as above, and the remaining mycelia can be used for pharmaceutical purposes.
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